The Human Hace1 Mouse Monoclonal Antibody (8D3) - Purified from MyBioSource.com is a Mouse Monoclonal antibody. The Human Hace1 Mouse Monoclonal Antibody (8D3) - Purified has been validated for the following applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot.
Description
Background: HACE 1 (HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1) is an ubiquitin ligase with six N-terminal ankyrin and a C-terminal HECT domain. HACE 1 is ubiquitously expressed in normal tissues and is frequently downregulated in human tumors. HACE 1 has been reported to act as a tumor suppressor through a phosphorylation-dependent degradation of cyclin D1. HACE1 has also been shown to bind GTP-bound Rac1 (Ras-related C3 botulinum toxin substrate 1) and to catalyze its polyubiquitination. HACE1 expression increases the ubiquitination of Rac1 when the GTPase is activated. HACE1 is required for cytotoxic necrosis factor 1 (CNSF1)-mediated depletion of Rac1 and efficient invasion of endothelial cell monolayer by bacteria, which suggests a role for HACE1 in host defense against pathogens. More recently, HACE1 was reported to target the Golgi membrane through interactions with Rab proteins. During mitotic Golgi disassembly, the ubiquitin ligase activity of HACE1 is required for subsequent postmitotic Golgi membrane fusion.
Description: A highly specific and sensitive antibody against HACE1 protein. This antibody was raised against the full length human HACE1 recombinant protein